文章标题
 
 
PHENOTYPE COMPARISONS FOR TOP METABOLIC MODELS
 
文章内容

 

C57BL/6J mice with mutations in leptin (B6-ob, Strain 000632) or its receptor (B6-db, Strain 000697) are hyperglycemic at young ages, but blood glucose levels eventually return to normal due to pancreatic islet cell hypertrophy.  By contrast C57BLKS/J mice with the same mutations (such as BKS-db, Strain 000642) are hyperglycemic throughout life.  Males and females in each strain have similar phenotypes, including weight and glucose (Figure 1, Table 1).  C57BL/6J male mice fed a high fat diet (DIO-B6, Strain 380050) are obese but not overtly diabetic (Table 1).  Detailed phenotypes for each strain are available.

metabolic-models-compared

Figure 1 Non-fasted blood glucose measured every other week in BKS-dbB6-dbB6-ob male and female mice.

Table 1 Comparison of four metabolic disease models at 8 and 16 weeks of age.  Blood was collected from the submandibular route and analyzed using a OneTouch Ultra2 or UltraMini glucometer (Blood Glucose) or a Beckman Coulter AU680 chemistry analyzer (Serum Glucose, HbA1c).

      8 Weeks 16 Weeks
Sex Strain Strain # Body Weight (g) Blood Glucose (mg/dL) Serum Glucose (mg/dL) HbA1c (NGSP %) Body Weight (g) Blood Glucose (mg/dL) Serum Glucose (mg/dL) HbA1c (NGSP %)
Male B6-ob 000632 44.5 ± 3.1 295 ± 86 362 ± 72 7.9 ± 1.6 58.2 ± 3.6 149 ± 66 212 ± 46 8.1 ± 0.7
B6-db 000697 40.8 ± 3.1 315 ± 92 399 ± 122 7.8 ± 0.8 54.3 ± 3.8 266 ± 107 273 ± 82 10.7 ± 1.7
BKS-db 000642 39.7 ± 2.0 415 ± 101 560 ± 70 7.5 ± 1.0 53.2 ± 3.5 509 ± 90 754 ± 77 13.1 ± 1.3
B6-DIO 380050 26.4 ± 1.5 215 ± 39 5.7 ± 0.3 41.3 ± 3.6 249 ± 44 6.4 ± 0.4
Female B6-ob 000632 44.4 ± 3.1 306 ± 97 261 ± 47 6.7 ± 0.8 60.7 ± 4.2 156 ± 50 196 ± 51 6.4 ± 0.5
B6-db 000697 39.0 ± 3.8 278 ± 88 310 ± 108 7.4 ± 0.8 55.2 ± 3.9 251 ± 115 370 ± 197 8.5 ± 1.9
BKS-db 000642 38.6 ± 2.6 359 ± 101 551 ± 85 7.1 ± 0.8 51.8 ± 4.2 517 ± 73 752 ± 101 12.0 ± 1.9
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